Contact our expert

Mihail Tamarkin
Product Manager +7 (915) 1997911

Benchtop SST, SIP 7-30

The Applikon Bio Bench bioreactor range consists of four different Sterilizable-In-Place bioreactor systems with a total volume of 7 liter, 15 liter, 20 liter and 30 liter. Applications are in basic research and small scale production for both mammalian cells and micro-organisms. The Bio Bench range is a direct scale-up from the lab scale autoclavable bioreactors (the world standard in laboratory research). This eases scale-up and minimizes risks in process transfer to next volume level.  The small footprint guarantees that the minimal amount of bench space is occupied by the system. All systems are fully documented for cGMP usage and are supplied with an extensive documentation package to support validation.

biobench30L ezcontrol

Modularity

The standardized design of the systems offers the flexibility needed in R&D to adapt the system to the changing process demands. A wide range of standard modules is available to customize the systems to the specific demands of the customer. The modularity extends to the control tower that can be supplied with the systems. A wide range of add-on packs are available. These standard packs include pressure control, weight control, double gas inlet lines, CIP modules biomass sensors, perfusion systems and many more.

Control

Applikon offers a choice of ez-Control and i-Control process control systems. Both systems offer automated Sterilization procedures. The i-Control adds automated Clean-In-Place procedures to the Bio Bench systems. The Bio Bench bioreactors can also be used with control systems that are already in place at the customers site. The BioXpert software is used for data collection, data visualization  and advanced supervisory control actions.

Specification

Bioreactor type
7 liter Bio Bench
15 liter Bio Bench
20 liter Bio Bench
30 liter Bio Bench

Inner Diameter (mm)
162 mm
211 mm
211 mm
265 mm

Inner Height (mm)
325 mm
442 mm
607 mm
546 mm

Liquid Height (mm)
248 mm
300 mm
447 mm
413 mm

Ports Head Space section
5 x 16 mm
5 x 27 mm
5 x 27 mm
2 x 16 mm
5 x 27 mm
1 x 1.5"TC

Ports Bottom section
3 x DN 25
5 x DN25
5 x DN25
5 x DN25

Ports Head Plate		 1 x M30x1,
5 x 10 mm,
4 x 16 mm,
4 x 27 mm		 1 x 69 mm,
3 x 1.5"TC,
3 x 16 mm,
4 x 27 mm		 1 x 69 mm,
3 x 1.5"TC,
3 x 16 mm,
4 x 27 mm		 1 x 69 mm,
3 x 1.5"TC,
3 x 16 mm,
4 x 27 mm

Bottom Drain
0.5"TC
0.5"TC
0.5"TC
0.5"TC

Floor Space (WxD)
530 x 700 mm
530 x 735 mm
530 x 735 mm
443 x 406 mm

Height
900 mm
1300 mm
1470 mm
802 mm

Total Volume
7 liter
15 liter
20 liter
30 liter

Working Volume
5 liter
10 liter
15 liter
22.5 liter

Min. Working Volume
2 liter
4 liter
4 liter
7 liter

H/D Total
2.2
2.1
3.0
2.0

H-D Work Vol.
15
1.4
2.1
1.6

Design Pressure Reactor
3.1 barg
3.1 barg
3.1 barg
3.1 barg

Design Pressure Jacket
6.2 barg
6.2 barg
6.2 barg
6.2 barg

Max. Temperature Reactor
150 ºC
150 ºC
150 ºC
150 ºC

Configuration

Parameter cell culture microbial culture
Temperature Electrical heating and cooling water injection into jacket. Steam injection into jacket for sterilization.
SIP systems have a pneumatically operated wetpart.
Options:
Double heatexchanger (closed loop),
Single heatexchanger for steam,
Superheated water
Empty sterilization,
Full sterilization.		 Electrical heating and cooling water injection into jacket. Steam injection into jacket for sterilization.
SIP systems have a pneumatically operated wetpart.
Options:
Double heatexchanger (closed loop),
Single heatexchanger for steam,
Superheated water
PH Acid via CO2 gas rotameter with valve, alkali via fixed speed pump
Option:
MFC CO2,
Acid pump		 Acid and alkali liquids via fixed speed pump.
DO2 O2 and Air via rotameters, O2 with valve. Air @ 0.1 vvm, O2 @ 0.01 vvm
Optional:
MFC's for both gasses
N2 gas via rotameter or MFC
 O2 and Air via rotameters. O2 with valve. Air @ 2 vvm, O2 @ 0.6 vvm
Optional:
MFC's for both gasses
N2 gas via rotameter or MFC
Agitation 1 m/s tipspeed
1 Marine impeller vortex for suspension cells or 1 Marine impeller scoping for anchorage dendant cells.
 6 m/s tipspeed
2 or 3 rusthon impellers depending on aspect ratio of the tank. One impeller per 0.7 H/D at working volume.
Gas flow
Air and O2 sparge are present.
CO2 is present for cell culture.
Other gases are optional.		 0.1    Vvm
Air sparge 0.1 vvm
Air overlay 0.2 vvm
O2 sparge 0.01 vvm
O2 overlay 0.1 vvm
N2 sparge 0.1 vvm
N2 overlay 0.2 vvm
CO2 sparge 0.05 vvm
CO2 overlay 0.1 vvm
Separate overlay is an option		 2-3 vvm
Air sparge 2 vvm
Air overlay 0.5 vvm
O2 sparge 0.6 vvm
O2 overlay 0.3 vvm
N2 sparge 1 vvm
N2 overlay 0.3 vvm
Separate overlay is an option
Gas outlet Non jacketed condenser, gas preheater and exhaust filter.
Optional jacketed condenser.		 Non jacketed condenser, gas preheater and exhaust filter.
Optional jacketed condenser.
Sterilization setpoint 121 deg C 20 minutes
Sterilization of the tank and gas inlet and outlet line is automatic.  Addition lines are manual. Full automatic is an option with I-Control.		 121 deg C 20 minutes
Sterilization of the tank and gas inlet and outlet line is automatic.  Addition lines are manual. Full automatic is an option with I-Control.
Pressure Pressure control is an option.
0 - 1 barg		 Pressure control is an option. 0 - 1 barg
Culture modes Batch, Fed Batch, Perfusion Batch, Fed Batch, Continuous, Repeated fed batch.
Sparger type Poral. Ring  or L-type.
Headspace aeration Yes, combined filter (Second filter line optional) Yes combined with sparger filter. (Second filter line optional)
Integrity testing of filters No (optional) No (optional)
Liquid inlets 2 push valves as standard.
Alkali, inoculum.
Contained inlets and extra push valves are optional		 4 push valves as standard.Acid, Alkali, Antifoaming agent, inoculum.
Contained inlets and extra push valves are optional
Sensors Standard PH, DO2, Temperature, (optional pressure, level, foam, biomass, O2 gas, CO2 gas and others) Standard PH, DO2, Temperature, foam (optional pressure, level, OD, O2 gas, CO2 gas and others)
Draining Tank outlet with large bore diameter to allow CIP Tank outlet with large bore diameter to allow CIP
Tank aspect ration
Pilot systems only		 H/D=1 at working volume,
H/D=1.5 at total volume.		 H/D=2.2 at working volume,
H/D=3 at total volume.
Agitation mounting Pilot systems Top Bottom (Top is optional)
Agitation mounting Bench systems Top Top
Spareparts Sparepart/startup kit included Sparepart/startup kit included
Software
Ez-Control only		 BioXpert Lite BioXpert Lite