Bioreactors to disposable receptacles
CellReady 3L Bioreactor Platform
General
Eliminate the need to clean, autoclave and assemble your
traditional glass bioreactors and save days of downtime with the
Mobius CellReady 3L Bioreactor and the CellReady controller
platform.
This 3L single-use, stirred tank bioreactor platform allows you to
focus on experimentation and data analysis, getting you to results
faster.
The CellReady bioreactor platform comes with 6 bioreactors,
which can be used for cell cultures (animal-, insects-, and plant
cells). The control system is formed by the standard Applikon
ez-Controller. The ez-Control system is configured for a cell
culture application, with rotameters and solenoids for Air, O2 and
CO2. It can measure and control pH, temperature, dissolved oxygen
(DO). Reusable sensors are provided for pH, temperature and
dissolved oxygen.
The bioreactor is equipped with multiple addition-, sample- and
harvesting tubing. All tubings are clear c-flex, suitable for tube
welding. Multiple diameter sizes tubing are used provided for
smaller and larger additions. Each line is also provided with luer
lock connector. The sample ports are provided with luer lock
connector. Four gas lines are present: gas inlet overlay, open pipe
sparger, porous sparger and exhaust gas. All lines are provided
with sterile (vent) filter.
The platform comes with a stirrer motor and adapter, a heating
blanket, sensors and cables, a liquid addition bottle for alkali
addition, tubing and a starter kit. The starter kit includes
several clamps, reducers and syringes for sampling.
Add-on packages are available to upgrade the system with MFC's,
extra pumps, etc.
Applications
The CellReady bioreactor platform can be used for cell cultures
(animal-, insects-, and plant cells).
Specifications
The CellReady 3L Bioreactor.
- Is the first, single-use 3L stirred tank bioreactor
- Comes pre-assembled and gamma irradiated
- Is compatible with your existing controller using our motor
adaptor - no capital equipment purchase
- Has a familiar, rigid tank design - no bags needed
- Provides instant gratification - just take it off the shelf and
start your experiment
The overall assembly includes:
- Multi-port Headplate
- Vessel (Semi-hemispherical bowl)
- Probe fittings (PG13.5)
- Impeller shaft with integrated bearing seal
- Marine impeller- Scoping (up-pumping)
- Base- removable and recyclable
- One way check valves
- CLC2000® Luer actuated sample ports
- Silicon seals- for bearing seal and headplate
- C-Flex® Tubing
- Vent Filter
- Millex® 33 inlet filters
- Tie wraps
- Pinch clamp
- Tubing connectors
- Male Luer-lock fittings
The Cellready platform controller.
- Is a standard Applikon ez-Control unit configured for cell
culture
- Comes with 3 rotameters with solenoid valves for control of pH
and dissolved oxygen content
- Comes with 1 pump for alkali addition
- Comes with a heating blanket for temperature control
- Can be fitted with extra rotameters, mass flow controllers and
2 extra built in pumps
- Allows up to 17 extra analog and digital inputs and outputs
with the extended I/O board.
Publications:
Regine Eibl, Stephan Kaiser, Renate Lombriser and Dieter Eibl,
Appl Microbiol Biotechnol, Disposable bioreactors: the current
state-of-the-art and recommended applications in biotechnology,
published online 22 January 2010.
Abstract:
Disposable bioreactors have increasingly been incorporated into
preclinical, clinical, and production-scale biotechnological
facilities over the last few years. Driven by market needs, and, in
particular, by the developers and manufacturers of drugs, vaccines,
and further biologicals, there has been a trend toward the use of
disposable seed bioreactors as well as production bioreactors.
Numerous studies documenting their advantages in use have
contributed to further new developments and have resulted in the
availability of a multitude of disposable bioreactor types which
differ in power input, design, instrumentation, and scale of the
cultivation container. In this review, the term "disposable
bioreactor" is defined, the benefits and constraints of disposable
bioreactors are discussed, and critical phases and milestones in
the development of disposable bioreactors are summarized. An
overview of the disposable bioreactors that are currently
commercially available is provided, and the domination of
wave-mixed, orbitally shaken, and, in particular, stirred
disposable bioreactors in animal cell-derived productions at cubic
meter scale is reported. The growth of this type of reactor system
is attributed to the recent availability of stirred disposable
benchtop systems such as the Mobius CellReady 3 L Bioreactor.
Analysis of the data from computational fluid dynamic simulation
studies and first cultivation runs confirms that this novel
bioreactor system is a viable alternative to traditional cell
culture bioreactors at benchtop scale.