AutoTrace 280 Solid-Phase Extraction Instrument
The AutoTrace® 280 SPE instrument is a powerful,
high-throughput workstation dedicated specifically for automating
Solid-Phase Extraction (SPE). The AutoTrace workstation automates
the cartridge or disk conditioning, sample loading, rinsing, and
eluting steps for large volume aqueous solution extractions (20 mL
to 20 L).

The unit can process up to six samples in parallel,
automatically conditioning, washing, and eluting SPE cartridges or
disks with a choice of up to five reagents. The unit also
segregates aqueous and solvent waste using separate waste lines for
efficient, cost effective waste handling.
Features
- Use SPE technology for liquid-liquid extraction
- Automated sample loading of liquids onto SPE cartridges
- Automated eluting of SPE cartridges with organic solvent
- Closed systems with a fan to vent solvent vapors
- Positive pressure loading and elution of samples and
solvents
Benefits
- Reduced solvent usage and elimination of glassware
- Does not require technician involvement to maintain a reservoir
of liquid in the SPE cartridge
- Does not require technician involvement to control the flow of
organics solvent through the SPE cartridge
- No hood required
- Provides constant flow of liquids through SPE cartridges
The AutoTrace systems are designed to automate the cartridge or
disk requirements for U.S. EPA water analysis methods. The
cartridge system is compatible with 1, 3, and 6 mL cartridges. The
disk system is compatible with 47 mm SPE disks.
AutoTrace Instrument Operation

AutoTrace systems provide solutions for many industries
Environmental
- Pesticides and herbicides
- PAHs and semi-volatiles
- PCBs
- Dioxins and furans
- Oil and grease
- Explosives
- Brominated flame retardants
- Urons (phenyl urea herbicides)
The AutoTrace instrument meets requirements for Solid Phase
Extraction for U.S. EPA 500 and 600 method series.
Pharmaceutical and Natural Products
- Active ingredients in liquids
- Extractables in packaged water
- Extractables in drug delivery devices and packaging
Food and Beverage
- Vitamins and antibiotics
- Contaminants in bottled drinks
- Natural products
- Pesticides and herbicides in liquids
- Pesticide residues
- Herbal and dietary supplements
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Perfomance
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Pesticide Recovery
Study N=6
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AutoTrace SPE
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Vacuum Manifolder
SPE
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Compound
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Recovery %
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% RSD
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Recovery %
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% RSD
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Atrazine
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88
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1,8
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54
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12,2
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Propazine
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91
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1,5
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80
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7,3
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Alachlor
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99
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3,4
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96
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4,1
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Metachlor
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99
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4,3
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96
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2,9
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SolEx Solid Phase Extraction Cartridges
The SolEx™ family of Solid-Phase Extraction (SPE) cartridges are
designed for fast and easy pre-concentration of contaminants prior
to analysis. SPE is one of the most important techniques for sample
enrichment because it overcomes many of the disadvantages of
liquid-liquid extraction.
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AutoTrace 280 Solid Phase
Extraction Instrument Specifications
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Gas Regulator and Gas Gauge Range:
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Output: 0-30 psi (0-1.4 bars),
Input: 100 psi (6.9 bars) maximum
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Net Weight:
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150 lbs. (68.1 kg)
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Dimensions (h × w × d):
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23 × 25 × 27 in (57 × 63.5 × 69 cm)
Sample Rack: 8 × 16.5 × 13 in
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500 & 600 Series EPA Methods:
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24 methods stored in the AutoTrace instrument,
unlimited number of methods stored in PC
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Electrical:
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Voltage: 100, 120, 220, or 240 V ± 10%
Frequency: 47-63 Hz
Power: 150 Volt AC
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Liquid Management:
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Air Syringe: One 10 mL air syringe
LH Syringe: One 10 mL liquid handling syringe
12-port Valve: Rotary, sliding Rulon® seal
Valves: 3-way, PTFE
Nozzles: Stainless steel
Tubing: PTFE, 1/16" ID, (6 total supplied)
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Sample Pumps:
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Displacement: Positive
Accuracy: ± 2.5%
Tube Fitting: Kynar®
Piston and Liner: Ceramic
Non Use: Not for use with acetic acid and acetone
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Solid Phase Extraction Configurations:
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1 mL Syringe: Compatible cartridge
3 mL Syringe: Compatible cartridge
6 mL Syringe: Compatible cartridge
47 mm SPE disk
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Rack and Collection Vial
Sizes
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Max. Solvent
Volumes
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15 mL conical tubes
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14 mL
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11 mm GC vials
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1.5 mL
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4 mL screw cap vials
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3.5 mL
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17 x 60 mm vials
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7.5 mL
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16 x 100 mm test tubes
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11 mL
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Ordering Information
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Product
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Cat.N
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AutoTrace Automated Large Volume SPE for Disks, 47mm
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071386
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AutoTrace Automated Large Volume SPE for Cartridges 6 mL
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071385
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AutoTrace Automated Large Volume SPE for Cartridges 3 mL
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072605
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AutoTrace Automated Large Volume SPE for Cartridges 1 mL
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072604
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AutoTrace Automated Large Volume SPE for Cartridges 6 mL
glass
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072606
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